Gen3 4U Value 10-Slot - GPU Expansion

can you use pcie 4 0 gpu in 3 0 slot
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In general, as long as you are able to run your GPU at PCI-e x8 or above , you can expect

With PCIe you get roughly 2 GBs of bandwidth per lane, giving the 6500 XT a ~8 GBs communication link with the CPU and system memory But

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can you tell when a slot machine will hit_ My question is: How using Gen VGA in Gen x8 slot may affect the performance I don't talk about gaming here Interested only in